Human Nischarin (NISCH) ELISA Kit from MyBioSource.com

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Human Nischarin (NISCH) ELISA Kit

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Description

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Nischarin (also known as NISCH) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human NISCH antibody. NISCH present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human NISCH Antibody is added and binds to NISCH in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NISCH antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human NISCH. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm